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tr 1003 phosphatase inhibitors topscience
Tr 1003 Phosphatase Inhibitors Topscience, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tr+1003+phosphatase+inhibitors+topscience/Anti-PHOSPHO1+Antibody/pm37865914-164-177-180
Average 93 stars, based on 16 article reviews
Tr 1003 Phosphatase Inhibitors Topscience, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tr+1003+phosphatase+inhibitors+topscience/Anti-PHOSPHO1+Antibody/pm37865914-164-177-180
Average 93 stars, based on 16 article reviews
tr 1003 phosphatase inhibitors topscience - by Bioz Stars,
2026-09
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Real-time Polymerase Chain Reaction:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# shRNA:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Sequencing:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Recombinant:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Software:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Modification:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Infection:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# SYBR Green Assay:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Protein Extraction:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Lysis:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Bicinchoninic Acid Protein Assay:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Reverse Transcription:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# Transfection:Article Title: Targeting VCP potentiates immune checkpoint therapy for colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: BALB/c (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000020-1 Mouse: C57BL/6J (female, age: 4–6 weeks) GemPharmatech Co. (Jiangsu, China) Cat# N000013 Oligonucleotides qPCR primers, see Table S1 This paper N/A shRNA and siRNA sequence, see Table S1 This paper N/A Recombinant DNA pCMV-33Flag-Puro OBiO Cat# GL119 pCMV-33Flag-VCP- Puro OBiO Cat# H18478 pLenti-Tet-On-shNC-Puro OBiO Cat# Y8938 pLenti-Tet-On-shVCP-1-Puro OBiO Cat# Y8936 pLenti-Tet-On-shVCP-2-Puro OBiO Cat# Y8937 pcDNA3.1(+)-HA Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-HA-Ubiquitin Laboratory of Prof. Mong-Hong Lee N/A pcDNA3.1(+)-Flag-JAK1 This paper N/A Software and algorithms Graphpad Prism 8.0 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://ImageJ.nih.gov/ij/ Image Lab Bio-Rad Laboratories https://www.bio-rad.com/ GSEA GSEA software http://www.gsea-msigdb.org/gsea/index. jsp CytExpert BECKMAN COULTER https://www.beckmancoulter.cn/ NIS-Elements Nikon N/A Zen Zeiss https://portal.zeiss.com/ Other Fetal Bovine Serum Thermo Fisher Scientific Cat# 1099141 Dulbecco’s Modified Eagle Medium Corning Cat# 10-013-CVR RPMI 1640 Medium Thermo Fisher Scientific Cat# C11875500BT 100X Penicillin-Streptomycin solution HyClone Cat# SV30010 Opti-MEM Thermo Fisher Scientific Cat# 31985070 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200072 Protease Inhibitors Topscience Cat# C0001 Polybrene Infection/Transfection Reagent Sigma-Aldrich Cat# |